As part of the VarioPore project, the Deigner working group will produce nanopores using electroporation and test their suitability for nanopore-based diagnostics using electroion beams. Nanopores will be chemically surface-modified for this purpose. Cultivated B. burgdorferi and its characteristic molecules such as surface proteins and antibodies are used as test objects. Finally, the measurement results will be compared with clinical samples and compared using PCR analyses and ELISA.